Amaranthus caudatus Lectin (ACL/ACA) - Texas Red

Affinity-purified Amaranthus caudatus Lectin (ACL/ACA) is a dimeric glycoprotein composed of two subunits. It preferentially binds to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc and can tolerate sialic acid substitutions at the 3 positions of galactose in the "T" antigen. While ACL does not exhibit specificity for blood groups, it shows a higher affinity for type O erythrocytes compared to types A and B.

Texas Red is a red-fluorescent dye and when bound to Amaranthus caudatus Lectin (ACL/ACA) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Recommended Usage: Recommended dilution is 0.5 – 10 µg /ml in 1XPBS. Agglutinate human, goat, sheep, and rabbit erythrocytes (1 – 10 µg /ml).

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Biomarker, Glycobiology

References

1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.