Artocarpus integrifolia Lectin (AIA) - Cy5

Jacalin lectin (AIA) is purified by affinity chromatography and features a tetrameric two-chain structure composed of four identical protomers, with an isoelectric point of pH 7.8. This lectin specifically targets blood group types O (+SA) and T and is recognized as a galactose-specific lectin, eluting with galactose or melibiose. A post-translational proteolytic modification at the N terminus of the α-chain creates a novel carbohydrate-binding site, which underlies its specificity for the T-antigen disaccharide Galβ1,3GalNAc. Inhibitors of jacalin include D-galactose, β-Met-Gal, and 2-deoxy-α-D-galactose.

Cy5 is conjugated to AIA to visualize the binding pattern of this lectin in cellular imaging and flow cytometry; its excitation wavelength is sufficiently high to prevent overlap with most other fluorochromes, facilitating dual-labeling experiments while minimizing background autofluorescence in biological specimens.

Recommended Usage: Recommended dilutions 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Mitogenic Stimulation, Cell selection.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. Mohamad SFS et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.: Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. VIJAYAKUMAR T and FORRESTER JA. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.