Bauhinia purpurea Lectin (BPL/BPA) - FITC (Fluorescein)

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding is generally highest for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures, but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind to BPL. BPA is lactose-specific and elutes with sugar lactose. It has specificity for blood groups A, B, and O (-SA). Treatment of erythrocytes with neuraminidase or trypsin increases the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. Although the binding specificity of BPA is similar to that of peanut agglutinin, the tissue staining patterns of these two lectins are distinct.

Affinity purified Bauhinia purpurea lectin (BPL/BPA) (SKU: 21510028) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide optimum staining characteristics. FITC conjugates have been used in various immunohistochemical and flow cytometry applications. This product comes in a stabilized liquid form.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate human or rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Blotting, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Glycobiology

References

1. N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
2. Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
3. Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
4. Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.