Bauhinia purpurea Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding is generally highest for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures, but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind to BPL. BPA is lactose-specific and elutes with sugar lactose. It has specificity for blood groups A, B, and O (-SA). Treatment of erythrocytes with neuraminidase or trypsin increases the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. Although the binding specificity of BPA is similar to that of peanut agglutinin, the tissue staining patterns of these two lectins are distinct.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. It is commonly used for blotting, immunoassays, and immunohistochemistry methods. Bauhinia purpurea lectin (BPL/BPA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate human or rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Bioconjugations, ELLISA, ELLA, Blotting, Biomarker, Glycobiology.

References

1. N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
2. Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
3. Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
4. Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.