Glycine max Lectin (SBA) - Pure

Glycine max agglutinin (SBA) is isolated and purified by affinity chromatography. It consists of four subunits of identical sizes. The isoelectric point of SBA is pH 6.0, binds to α/β N-acetylgalactosamine and gfalactose. SBA is a 120 kDa glycoprotein. SBA has specificity for blood groups A1, A2, and B, and interacts better with neuramidase-treated cells than untreated cells. This Lectin has selective affinity for lymphocytes and human CD34+ hematopoietic stem cells. Immobilized conjugates of SBA are important tools for removing T-cells in bone marrow transplants.

Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS. Neuraminidase treated erythrocytes will agglutinate by 1 – 10 μg / ml of SBA.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Stem cell separation, Bioconjugation, Immunofluorescence, Blotting, Glycobiology.

References

1.A. M. Calder6n de la Barca, L. Vhzquez-Moreno, & M. R. Robles-Burguefio. Active Soybean Lectin in Foods: Isolation and Quanfitation. Food Chemistry 39 (1991) 321-327.
2.Gade W, Jack MA, Dahl JB, Schmidt EL, Wold F. The isolation and characterization of a root lectin from soybean (Glycine max (L), cultivar Chippewa). J Biol Chem. 1981 Dec 25;256(24):12905-10.
3.Amit T Bondar, Shobha B Tole and Dr. Shivanand M Patil. Isolation and Purification of Lectin from Soybean (Glycine Max) Mahabeej Js-335. Journal of Pharmacognosy and Phytochemistry 2016; 5(6): 89-92.