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Description

Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Lens culinaris (Lentil) Lectin (LCA/LCH) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Off-white to brown

Appearance form

Liquid

Molecular Weight

49 kDa

Solubility (@ RT)

1 mg/ml in PBS at pH 6.8

Abbreviation (Lectins Only):

LCA/LCH

Source

Lentil

Blood Group Specificity

Human and Rabbit Erythrocytes, Lymphocytes

Carbohydrate Specificity

D-Mannose > D-Glucose

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Biotin

Inhibitory Carbohydrate

D-Mannose > D-Glucose

Divalent Ions

Ca++, Mn++

Cancer Biomarker

Hepatocellular Cancer