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Description

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Lens culinaris (Lentil) Lectin (LCA/LCH) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Orange to brown

Appearance form

Liquid

Molecular Weight

49 kDa

Solubility (@ RT)

1 mg/ml in PBS at pH 6.8

Abbreviation (Lectins Only):

LCA/LCH

Source

Lentil

Blood Group Specificity

Human and Rabbit Erythrocytes, Lymphocytes

Carbohydrate Specificity

D-Mannose > D-Glucose

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Horseradish Peroxidase (HRP)

Inhibitory Carbohydrate

D-Mannose > D-Glucose

Divalent Ions

Ca++, Mn++

Cancer Biomarker

Hepatocellular Cancer