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Description

Fractionated Lens culinaris lectin (LCA) is composed of four subunits that consists of an ?-chain (MW: 5.7 kDa) and ?-chain (MW: 17.5 kDa). It has a carbohydrate specificity toward ?-D-mannose and ?-D-glucose, eluting with the sugar Me?Man+Me?Glc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8�g/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Appearance form

Lyophilized Powder

Molecular Weight

46 kDa

Abbreviation (Lectins Only):

LCA/LCH

Source

Lentil

Blood Group Specificity

Non-specific

Carbohydrate Specificity

α-Mannose, α-Glucose

Conjugate/Tag/Matrix

None

Inhibitory Carbohydrate

α-Methylmannoside + α-Methylglucoside

Divalent Ions

Ca++, Mn++

Cancer Biomarker

Hepatocellular Cancer

Mitogenic Activity

Yes