Lycopersicon esculentum Lectin (LEL/LEA) - Cy3

Lycopersicon esculentum lectin (LEL/LEA) is Isolated and affinity-purified from tomatoes, has carbohydrate specificity for GlcNAc. Composed of a single stable subunit, LEL serves as a valuable marker for tracking vasculature in rodents and neuroscience research. With an unusually high carbohydrate content of about 50 percent arabinose and galactose, LEL non-specifically agglutinates human erythrocytes of blood types A, B, O, and AB. Protease-treated erythrocytes show slightly increased sensitivity to agglutination by this lectin.

Cy3 is used to visualize the binding pattern of Lycopersicon esculentum Lectin (LEL/LEA) in cellular imaging and flow cytometry. It offers greater photostability than many other fluorophores and is compatible with TRITC filter sets. Cy3 is often combined with green-fluorescent dyes for effective dual-labeling applications.

Recommended usage: Recommended dilutions of 0.5-10 μg/ml in 1XPBS. To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at (-20⁰C).

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Cell sorting,

References

1.Naito Y, Minamihara T, Ando A, Marutani T, Oguri S, Nagata Y. Domain construction of cherry-tomato lectin: relation to newly found 42-kDa protein. Biosci Biotechnol Biochem. 2001 Jan;65(1):86-93.
2.Keiko SAITO, Hiromi MAGI, Kei'ichi BABA, Irwin J. GOLDSTEIN and Akira MISAKI Oyo. Purification, Properties and Carbohydrate-Binding Specificity of Cherry Tomato (Lycopersion esculentum var. Cherry) Lectin Toshitsu Kagaku (J. Appl. Glycosci.), Vol. 43, No. 3, p. 331-345 (1996).