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Description

Affinity-purified Lycopersicon esculentum (Tomato) Lectin (LEL/LEA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.

Tomato Lectin (LEL, TL) is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose. FITC labeled LEL binds to glycophorin and Tamm-Horsfall glycoprotein and has been used to label vascular endothelium in mouse and rats.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

IHC
Flow cytometry

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

Affinity purified

Appearance color

Yellow to orange

Appearance form

Liquid

Molecular Weight

71 kDa

Abbreviation (Lectins Only):

LEL/LEA

Source

Cherry Tomato

Blood Group Specificity

Rabbit, Human Erythrocytes

Carbohydrate Specificity

GlcNAc

Storage Buffer

PBS pH 7.4, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

FITC (Fluorescein)

Inhibitory Carbohydrate

(GlcNAc)5

Fluorescence

Green

Divalent Ions

None Required

Excitation

498 nm

Emission

517 nm