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Description

Alkaline phosphatase (AP) is conjugated to Maclura pomifera Lectin (MPL/MPA) to show the binding of MPL/MPA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Clear, Colorless to Light Brown

Appearance form

Liquid

Molecular Weight

40 kDa

Abbreviation (Lectins Only):

MPL/MPA

Source

Osage Orange Seeds

Blood Group Specificity

Human Erythrocytes, T Lymphocytes

Carbohydrate Specificity

GalNAc, Melibiose, Galactose

Storage Buffer

PBS pH 7.4, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Alkaline Phosphatase (AP)

Inhibitory Carbohydrate

Methyl α-D-Galactopyranoside

Divalent Ions

None Required

Mitogenic Activity

Yes