Musa paradisiaca Lectin (BanLec) - HRP (Horseradish Peroxidase)

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units.

A buffer washed column elutes the sugar Methyl α-Mannopyranoside.
Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.

Recommended Usage: Recommended dilutions of 1-20 μg/ml in 1XPBS. 1-20 μg/ml of NPL can agglutinate rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Bioconjugation, Blotting, Cancer biomarker, Early apoptotic stage detector, Cell typing, Glycobiology.

References

1.Lopez S, Codina C, Bastida J, Viladomat F, Davidson E, Stewart D. Biodiversity of mannose-specific lectins within Narcissus species. J Agric Food Chem. 2002 Apr 24;50(9):2507-13.
2.Kaku H, Van Damme EJ, Peumans WJ, Goldstein IJ. Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins. Arch Biochem Biophys. 1990 Jun;279(2):298-304.