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Description

Cy5, when bound to Phaseolus vulgaris Lectin (PHA-L), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Appearance color

Blue to purple

Appearance form

Liquid

Molecular Weight

130 kDa

Abbreviation (Lectins Only):

PHA-L

Source

Red Kidney Bean

Blood Group Specificity

Human, Rabbit Leukocytes

Carbohydrate Specificity

Galactose

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Cyanine 5 (Cy5)

Inhibitory Carbohydrate

Neu5Ac?2-3Gal?1-4GlcNAc?1-2Man

Fluorescence

Far red

Divalent Ions

Ca++, Mn++

Excitation

647 nm

Mitogenic Activity

Yes

Emission

665 nm