Styphnolobium japonicum Lectin (SJA) - Semi-Pure

Styphnolobium japonicum agglutinin (SJA) is fractionated from Japanese pagoda tree seeds. It consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric point between pH 4.9 and pH 5.6 and a carbohydrate specificity towards ?GalNAc. This lectin elutes with the sugar GalNAc.
SJA agglutinates all blood group types but has greater affinity for A erythrocytes than of B types, than of O (-SA) types. Binding experiments of the lectin with frozen sections of human kidneys shows specific binding to the endothelia in specimens from blood groups B or AB, thus indicating a D-galactose/N-acetyl-D-galactosamine receptor specificity. SJA lacks mitogenic and immune-suppressive activity.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C