Succinylated Concanavalin A Lectin (Succ Con A) - Pure

Affinity-purified Succinylated Concanavalin A (Succ-Con A) is derived from native concanavalin A (Con A). This non-agglutinating lectin specifically targets α-linked mannose and glucose, eluting with α-methylglucoside and α-methylmannoside. Succ-Con A has been investigated for its ability to significantly stimulate the proliferation of human peripheral blood lymphocytes, demonstrating mitogenic activity comparable to that of native Con A, and both enhance lymphocyte levels of cyclic GMP. Unlike Con A, Succ-Con A maintains its mitogenic activity even at high concentrations. Additionally, it reversibly inhibits the growth of normal cells while restoring a normal growth phenotype in transformed cells.

Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in Tris Buffer (20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2). Succ Con A is relatively weak blood agglutinin. More than 10 ug/ml may be required to give visible agglutination of neuraminidase treated human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Bioconjugation, Blotting, ELISA, Cell typing, Glycobiology.

References

1.Homa Darmani and w. Terence Coakley. Contact Patterns in Concanavalin A Agglutinated Erythrocytes. Cell Biophysics, Vol. 18, 1991.
2.McCubbin WD, Kay CM. Molecular weight studies on concanavalin A. Biochem Biophys Res Commun. 1971 Jul 2;44(1):101-9.
3.Olson MO, Liener IE. Some physical and chemical properties of concanavalin A, the phytohemagglutinin of the jack bean. Biochemistry. 1967 Jan;6(1):105-11.