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Description

Alkaline phosphatase (AP) is conjugated to Succinylated Triticum vulgaris Lectin (Succ WGA) to show the binding of Succ WGA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Clear, Colorless to Light Brown

Appearance form

Liquid

Molecular Weight

36 kDa

Abbreviation (Lectins Only):

Succ WGA

Source

Wheat

Blood Group Specificity

Rabbit, Human Erythrocytes

Carbohydrate Specificity

Chitin, GlcNAc

Storage Buffer

PBS pH 7.4, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Alkaline Phosphatase (AP)

Inhibitory Carbohydrate

(GlcNAc)5

Divalent Ions

None Required

Mitogenic Activity

No