Wisteria floribunda Lectin (WFA/WFL) - Pure

Wisteria floribunda agglutinin or lectin (WFA/WFL) is isolated from Japanese Wisteria seeds by affinity chromatography. It is a glycoprotein with a molecular weight of 68 kDa and is composed of four subunits. It has an isoelectric point between pH 5.2 and pH 5.8. There is no blood group specificity for this Lectin, although it does react stronger with human type A1 cells over A2, B, or O cells. The presence of a sialic acid residue may explain the Lectins lack of reactivity. The increased reactivity of neuraminidase treated erythrocytes with WFA supports this conclusion.

WFA contains a strong hemagglutinin activity and is a potent mitogen, and strongly inhibited by GalNAc, although mitogenic activity is also inhibited by N,N’-di-acetylchitobiose. Isolated by use of ion-exchange chromatography, and affinity gel filtration.

Recommended Usage: Recommended dilution of 0.5 – 10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2.  Less than 20 μg/ml of WFA can agglutinate human/porcine erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Blotting, Bioconjugations, Diagnostic tools for bioassays, Cell typing, Glycobiology.

References

1. Haji-Ghassemi O, Gilbert M, Spence J, Schur MJ, Parker MJ, Jenkins ML, Burke JE, van Faassen H, Young NM, Evans SV. Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[β1→4]GlcNAc), by Wisteria floribunda Agglutinin. J Biol Chem. 2016 Nov 11;291(46):24085-24095.
2. Kurokawa T, Tsuda M, Sugino Y. Purification and characterization of a lectin from Wistaria floribunda seeds. J Biol Chem. 1976 Sep 25;251(18):5686-93.
3. Cheung G, Haratz A, Katar M, Skrokov R, Poretz RD. Purification and properties of the hemagglutinin from Wistaria floribunda seeds. Biochemistry. 1979 May 1;18(9):1646-50.