CAPS Buffer 0.5M, pH 10.0

Synonym: 3-(Cyclohexylamino)-1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

• CAPS buffer is a zwitterionic buffer widely used in Western and immunoblotting experiments as well as protein sequencing and identification.
• Used in the electrotransfer of proteins to PVDF or nitrocellulose membranes.
• The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS buffer has very low metal-binding constants and are therefore particularly suited to investigate metal-dependent enzymes.
• CAPS is selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Technical Information:

pKa (25°C): 10.40

Warning: For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.

• Appearance: Liquid
• Density: 1.20 g/cm3 (Predicted)
• Melting Point: >300 °C(lit.)
• PH Value: 10.0
• RTECS Number: TZ6395000