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Description

Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose--1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Pure Lectin was conjugated with Alexa Fluor™ 488, is a bright green, fluorescent dye with an excitation 488 to maximum emission 519 nm. The ABA Alexa Fluor™ 488 conjugate is ideal for immunofluorescence and immunohistochemistry, immunocytochemistry. Alexa Fluor™ 488 dye is water soluble and pH-insensitive (pH 4 to pH 10), ensuring reliable performance across various conditions. This lectin-dye conjugate is optimized for cellular labeling and detection, making it a valuable tool for enhancing sensitivity in research applications.

Application: Glycobiology, Immunohistochemistry, immunofluorescence.

Recommended usage: Recommended concentrations 5 - 20 ug/ml in 1XPBS.

 

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

 

Properties

Shelf life

2 years

Storage Temperature

-20°C

Appearance color

Yellow/orange

Appearance form

Liquid

Molecular Weight

64 kDa

Abbreviation (Lectins Only):

ABA/ABL

Source

White Button Mushroom

Blood Group Specificity

Human, Rabbit Erythrocytes

Carbohydrate Specificity

Lactose, Galβ1–3GalNAc

Storage Buffer

PBS pH 7.4, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Alexa Fluor 488

Inhibitory Carbohydrate

GlcNAc, Galβ1–3GalNAc

Fluorescence

Bright Green

Divalent Ions

None Required

Excitation

490 nm

Mitogenic Activity

Emission

525 nm