Agaricus bisporus Lectin (ABA/ABL) - Alexa Fluor 647

Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose--1,3-N-acetylgalacto samine (Gal--1,3-GalNAc; also known as T-antigen disaccharide) and galactose--1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins. Pure Lectin was conjugated with Alexa Fluor™ 647 is a far-red fluorescent dye with an excitation 650 to maximum emission 671 nm . The ABA Alexa Fluor™ 647 conjugate is ideal for immunofluorescence, immunohistochemistry and immunocytochemistry. Alexa Fluor™ 647 dye is water soluble, photo and  pH-stable (pH 4 to pH 10), ensuring reliable performance across various conditions. This lectin-dye conjugate is optimized for cellular labeling and detection, making it a valuable tool for enhancing sensitivity in research applications.

Application: Glycobiology, Immunofluorescence, Immunohistochemistry, Immunocytochemistry

Recommended Usage: Recommended concentration 5-20 ug/ml in 1XPBS.