Agaricus bisporus Lectin (ABA/ABL) - Texas Red

Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.

Texas Red is a red-fluorescent dye bound to Agaricus bisporus Lectin (ABA/ABL) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Application: Immunofluorescence, Immunohistochemistry, Cell typing, Glycobiology

Recommended Usage: 1XPBS pH 7.4 can be used to dilute the stocks. 5-10 µg/ml will agglutinate type A or B cells. 2 µg/ml will agglutinate type O cells. Less than 0.5 µg/ml will agglutinate neuraminidase treated erythrocytes.

Recommended Usage: 1XPBS pH 7.4 can be used to dilute the stocks. 5-10 µg/ml will agglutinate type A or B cells. 2 µg/ml will agglutinate type O cells. Less than 0.5 µg/ml will agglutinate neuraminidase treated erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C