Allium sativum Lectin (ASA) - Semi-Pure

Allium sativum semi pure lectin (ASA) is extracted from garlic bulbs. The lectin is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. The binding site can withstand terminal sialic acid and galactose residues. The blood group specificity of ASA is towards rabbit erythrocytes and weak interaction with human erythrocytes. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.

Recommended Usage: 1XPBS (pH 7.4) buffer can be used to reconstitute the Lyophilized Cake.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Cell typing, Glycobiology, Cancer biomarker (pure), Diagnostic tool.

References

1. Ghosh, P., Sen, S., Chakraborty, J., & Das, S. (2016). Monitoring the efficacy of utated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani. BMC biotechnology, 16, 24. https://doi.org/10.1186/s12896-016-0246-0.

2. Kumar, S., Jitendra, K., Singh, K., Kapoor, V., Sinha, M., Xess, I., Das, S. N., Sharma, S., Singh, T. P., & Dey, S. (2015). Biological Properties and Characterization of ASL50 Protein from Aged Allium sativum Bulbs. Applied biochemistry and biotechnology, 176(7), 1914–1927.