Amaranthus caudatus Lectin (ACL/ACA) - TRITC (Rhodamine)

Affinity-purified Amaranthus caudatus Lectin (ACL/ACA) is a dimeric glycoprotein composed of two subunits. It preferentially binds to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc and can tolerate sialic acid substitutions at the 3 positions of galactose in the "T" antigen. While ACL does not exhibit specificity for blood groups, it shows a higher affinity for type O erythrocytes compared to types A and B.

Amaranthus caudatus Lectin (ACL/ACA) is conjugated with tetramethylrhodamine isothiocyanate (TRITC) to enhance its staining characteristics. TRITC is a bright orange-red fluorescent dye, optimal for excitation at the 532-nm laser line. This TRITC-labeled ACL/ACA is suitable for cellular imaging applications.

Recommended Usage: Recommended dilution 0.5 – 10 µg/ml in 1XPBS. Agglutinate human, goat, sheep, and rabbit erythrocytes (1 – 10 µg /ml).

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell sorting, Biomarker, Glycobiology.

References

1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.