Artocarpus integrifolia Lectin (AIA) - Pure

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography and features a tetrameric two-chain structure composed of four identical protomers, with an isoelectric point of pH 7.8. This lectin is specific for blood group types O (+SA) and T. Jacalin is classified as a galactose-specific lectin, eluting with galactose or melibiose. A post-translational proteolytic modification introduces a novel carbohydrate-binding site at the N terminus of the α-chain. The protein's structure accounts for its binding specificity to the T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal, and 2-deoxy-α-D-galactose. This product is available in a stable lyophilized form.

Recommended Usage: Recommended dilutions 0.5 – 10 µg / ml in 1XPBS. Less than 0.5 µg / ml will agglutinate human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Cell typing, Western blotting, IgG purification. Bioconjugation. Cancer biomarker. AIDS Research. Glycobiology.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.