Artocarpus integrifolia Lectin (AIA) - TRITC (Rhodamine)

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography and consists of a tetrameric two-chain structure with four identical protomers and an isoelectric point of pH 7.8. The lectin specifically interacts with blood group types O (+SA) and T. Jacalin is classified as a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin introduces a novel carbohydrate-binding site involving the N terminus of the α-chain, contributing to its specificity for the T-antigen disaccharide Galβ1,3GalNAc. Potent inhibitors of jacalin include D-galactose, β-Met-Gal, and 2-deoxy-α-D-galactose.

 AIA is labeled with tetramethylrhodamine isothiocyanate (TRITC), providing optimal staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye, ideally excited at the 532-nm laser line, and is suitable for cellular imaging applications. This product is available in a stabilized liquid form.

Recommended Usage: Recommended dilutions of 0.5 - 10 µg / ml in 1XPBS. Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Blotting, Cell typing, Glycobiology.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.