Bauhinia purpurea Lectin (BPL/BPA) - AP (Alkaline Phosphatase)

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.

Alkaline phosphatase (AP) is conjugated to Bauhinia purpurea Lectin (BPL/BPA) to facilitate the visualization of BPL/BPA binding in various applications, including Western blotting and ELISA. Alkaline phosphatase is a large protein with a molecular weight of 140 kDa that catalyzes the hydrolysis of phosphate groups from substrates, resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein occurs between pH 8 and 10, and its reaction rate remains linear, enhancing sensitivity over time.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1XPBS. Less than 0.5 µg / ml will agglutinate human/rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Western blotting, ELISA, Immunohistochemistry, Immunocytochemistry, Glycobiology.

References

1. N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
2. Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
3. Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
4. Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.