Bauhinia purpurea Lectin (BPL/BPA) - Biotinylated

Affinity purified Bauhinia purpurea agglutinin (BPA/BPL) is a tetrameric Lectin with a molecular weight of 120 kDa. It has the highest binding affinity for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures and can also bind oligosaccharides with a terminal α-linked N-acetylgalactosamine. BPA is lactose-specific, eluting with lactose, and shows specificity for blood groups A, B, and O (−SA). Treatment with neuraminidase or trypsin enhances agglutination, indicating that the receptor is masked by terminal carbohydrates. N-acetyl-D-galactosamine from Makela's group 2 sugars acts as a potent inhibitor. While BPA's binding specificity resembles that of peanut agglutinin, their tissue staining patterns are distinct.

Biotinylated BPL/BPA features an optimal amount of biotin for enhanced detection characteristics when using Avidin-HRP or Streptavidin-HRP conjugates, facilitating more sensitive detection in ELISA and Western-blotting applications.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1X PBS. Less than 1 µg / ml will agglutinate human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Diagnostic tools, Glycan array, Blotting, Glycobiology.

References

1. N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
2. Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
3. Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
4. Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.