Concanavalin A Lectin (Con A) - Alexa Fluor 647

Concanavalin A Lectin (Con A) is isolated from jack beans (Canavalia ensiformis) and purified by affinity chromatography. Con A is composed of identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues) and while above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. It has a carbohydrate specificity towards α-mannose and α-glucose and elutes with MeαMan + MeαGlc. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells which aggregate by Con A (normal white cells do not). Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. This product comes in a stable lyophilized form.

Concanavalin A Lectin (Con A) conjugated with Alexa Fluor™ 647 is a bright, far red fluorescent dye with excitation ideally suited to the 633 nm laser line. This dye is optimized for stable signal generation in imaging and flow cytometry. The conjugate is water soluble and pH-insensitive from pH 4 to pH 10, ensuring consistent results. Alexa Fluor™ 647 facilitates effective cellular labeling and detection, making it an excellent option for advanced applications in biological research.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg ml in 1XPBS. More than 10 µg/mL may be required to give visible agglutination of neuraminidase treated human erythrocytes.

Application

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Glycan array, Flow cytometry, Golgi and ER marker, Dot blot, Glycobiology.

References

1.Darmani H, Coakley WT. Contact patterns in concanavalin A agglutinated erythrocytes. Cell Biophys. 1991 Feb;18(1):1-13.
2.Olson MO, Liener IE. Some physical and chemical properties of concanavalin A, the phytohemagglutinin of the jack bean. Biochemistry. 1967 Jan;6(1):105-11.
3.McCubbin WD, Kay CM. Molecular weight studies on concanavalin A. Biochem Biophys Res Commun. 1971 Jul 2;44(1):101-9.