Erythrina cristagalli Lectin (ECL/ECA) - Pure

Erythrina cristagalli lectin or agglutinin (ECL/ECA) is affinity purified lectin consists of two subunits with molecular weights of 28,000 and 26,000. ECL binds to carbohydrate structure in membrane and serum glycoproteins of mammalian cells. The shape of the lectin binding domains may resemble cavity type with Galβ1->4GlcNAc as the core binding site with additional one to four sugar subsites and is most complementary to a linear trisaccharide. Some Galβ1-related oligosaccharides are the major structures for lectin binding. Lectin binding activity can be abolition through sialic acid substitution. This allows specificity to fractionate and isolate mammalian glycoproteins.

Recommended Usage: Recommended dilutions of 0.5 – 10 µg/ml in 1XPBS. Neuraminidase treated human erythrocyte will agglutinate by 0.5 – 20 µg/ml of ECL.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Cell sorting, Bioconjugation, Pain management, ELLISA, Blotting, Cell surface analysis, Glycobiology.

References

1.Datta TK, Basu PS. Identification, isolation and some properties of lectin from the seeds of Indian coral tree [Erythrina variegata (Linn.) var. orientalis (Linn.) Merrill]. Biochem J. 1981 Sep 1;197(3):751-3.
2.Iglesias JL, Lis H, Sharon N. Purification and properties of a D-galactose/N-acetyl-D-galactosamine-specific lectin from Erythrina cristagalli. Eur J Biochem. 1982 Apr 1;123(2):247-52.