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Griffonia simplicifolia Lectin (GSL I+II) - AP (Alkaline Phosphatase)

21510726 bioPLUS™, GlycoMatrix™

Griffonia simplicifolia Lectin (GSL I+II) - AP (Alkaline Phosphatase)

liquid, high purity grade, from bandeiraea

Molecular Weight: 113 kDa

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  SKU Pack Size Price
21510726-1 1 mg - 1 mL $222.15


$222.15

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Description

Alkaline phosphatase (AP) is conjugated to Griffonia simplicifolia Lectin (GSL I+II) to show the binding of GSL I+II in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Clear, Colorless to Light Brown

Appearance form

Liquid

Molecular Weight

113 kDa

Abbreviation (Lectins Only):

GSL I+II

Source

Bandeiraea

Blood Group Specificity

A(-SA), B

Carbohydrate Specificity

GalNAc, Galactose, GlcNAc

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Alkaline Phosphatase (AP)

Inhibitory Carbohydrate

Methyl α-D-Galactopyranoside, Melibiose, N,N'-Diacetylchitobiose

Divalent Ions

Ca++, Mn++

Mitogenic Activity