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Description

Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Griffonia simplicifolia Lectin (GSL I+II) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

High Purity Grade

Appearance color

Off-white to brown

Appearance form

Liquid

Molecular Weight

113 kDa

Solubility (@ RT)

Soluble in 0.9% sodium chloride solution (1 m

Abbreviation (Lectins Only):

GSL I+II

Source

Bandeiraea

Blood Group Specificity

A(-SA), B

Carbohydrate Specificity

GalNAc, Galactose, GlcNAc

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

Biotin

Inhibitory Carbohydrate

Methyl α-D-Galactopyranoside, Melibiose, N,N'-Diacetylchitobiose

Divalent Ions

Ca++, Mn++

Mitogenic Activity