Hippeastrum hybrid Lectin (HHL) - Pure

Affinity purified Hippeastrum hybrid Lectin (HHL) from Amaryllis bulbs, consists of four identical subunits. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. This lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans. 

Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 1XPBS pH 7.4. Rabbit erythrocytes will agglutinate by 1-20 μg of HHL Lectin.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Blotting, ELLISA, Bioconjugation, Cancer bio marker, Cells studies, Antiviral research, Glycobiology.

References

1.ELS J. M. VAN DAMME, IRWIN J. GOLDSTEIN* and WILLY J. PEUMANS. A COMPARATIVE STUDY OF MANNOSE-BINDING LECTINS FROM THE AMARYLLIDACEAE AND ALLIACEAE. Phytochemistry, 1991, Vol. 30, No. 2, pp. m-514.