Lycopersicon esculentum Lectin (LEL/LEA) - Pure

Lycopersicon esculentum lectin (LEL/LEA) is Isolated and affinity-purified from tomatoes, has carbohydrate specificity for GlcNAc. Composed of a single stable subunit, LEL serves as a valuable marker for tracking vasculature in rodents and neuroscience research. With an unusually high carbohydrate content of about 50 percent arabinose and galactose, LEL non-specifically agglutinates human erythrocytes of blood types A, B, O, and AB. Protease-treated erythrocytes show slightly increased sensitivity to agglutination by this lectin.

Recommended Usage: Recommended dilutions of 0.5 10 μg/ml in 1XPBS pH 7.4. 50-60 µg/ml will agglutinate type O human erythrocytes. 5 -10 µg/ml will agglutinate neuraminidase treated cells.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Vascular Imaging, Blood vessels mapping in CNS, Bioconjugations, Cellular analysis, Diagnostic tools, Glycobiology.

References

1.Naito Y, Minamihara T, Ando A, Marutani T, Oguri S, Nagata Y. Domain construction of cherry-tomato lectin: relation to newly found 42-kDa protein. Biosci Biotechnol Biochem. 2001 Jan;65(1):86-93.
2.Keiko SAITO, Hiromi MAGI, Kei'ichi BABA, Irwin J. GOLDSTEIN and Akira MISAKI Oyo. Purification, Properties and Carbohydrate-Binding Specificity of Cherry Tomato (Lycopersion esculentum var. Cherry) Lectin Toshitsu Kagaku (J. Appl. Glycosci.), Vol. 43, No. 3, p. 331-345 (1996).