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Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. Its hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Purity

Affinity purified

Appearance color

Off-white to brown

Appearance form

Lyophilized Powder

Molecular Weight

50 kDa

Activity Level

Between 10-100 µg/mL may be required to agglutinate neuraminidase treated red blood cells.

Abbreviation (Lectins Only):

PSA/PSL

Source

Pea

Blood Group Specificity

Rabbit Erythrocytes

Carbohydrate Specificity

D-Mannose, D-Glucose

Inhibitory Carbohydrate

Methyl ?-D-Mannopyranoside, Trehalose

Divalent Ions

Ca++, Mn++

Mitogenic Activity

Yes