Sambucus nigra Lectin (SNA/EBL I) - Alexa Fluor 594

Sambucus nigra Lectin (SNA/EBL I) is an isolectin, isolated from elderberry bark and purified via affinity chromatography. As a type 2 ribosome-inactivating protein, it consists of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. SNA I specifically targets α2,6-linked sialic acid residues and elutes with lactose. At low concentrations, it can induce apoptosis through a caspase-dependent pathway, an effect that relies on the carbohydrate binding of the B-chain and is independent of the A-chain's catalytic activity. SNA I inhibit cell free protein synthesis.

Sambucus nigra Lectin conjugated with Alexa Fluor™ 594 is a bright red fluorescent dye used for stable signal generation in imaging and flow cytometry. These conjugate features optimal excitation for effective detection and is water soluble and pH-insensitive from pH 4 to pH 10. Alexa Fluor™ 594 offers superior brightness and photostability, making it an ideal choice for a variety of cellular labeling and detection applications.

Recommended usage: Recommended dilutions of 0.5 – 10 μg/ml in 1XPBS pH 7.4. To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20⁰C.

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell sorting, Blotting, ELISA, Biomarker, Glycobiology.

References

1. Broekaert WF, Nsimba-Lubaki M, Peeters B, Peumans WJ. A lectin from elder (Sambucus nigra L.) bark. Biochem J. 1984 Jul 1;221(1):163-9.
2. Kaku H, Peumans WJ, Goldstein IJ. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. Arch Biochem Biophys. 1990 Mar;277(2):255-62.
3. Maveyraud L, Niwa H, Guillet V, Svergun DI, Konarev PV, Palmer RA, Peumans WJ, Rougé P, Van Damme EJ, Reynolds CD, Mourey L. Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra. Proteins. 2009 Apr;75(1):89-103.
4. Mach L, Scherf W, Ammann M, Poetsch J, Bertsch W, März L, Glössl J. Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit. Biochem J. 1991 Sep 15;278 (Pt 3):667-71.