Lauryl Sulfate Broth

Lauryl Sulfate Broth

• For the detection of coliforms in water, wastewater and foods
• Prepared Appearance: tan to yellow broth

Use:

Dissolve 35.6 g per 1 L 3d-water. Distribute to convenient flasks or dispense 10 mL into each test-tubes. Sterilize by autoclaving at 15 minutes. Cool down slowly to prevent bubbles tubes.

Gas production from lactose fermentation is indicated by using inverted Durham tubes. Inoculate the tubes containing 10 mL of Lauryl sulfate Broth and the tubes. Incubate at the wished temperature for 18-24 hours. In case of gas formation the Durham tubes rise or/and show bubbles.

Turbidity of the medium accompanied by formation of gas within 48 hours is a positive presumtive test for the presence of E. coli and/or other coliform organisms.

mposition: