Allium sativum Lectin (ASA) - DyLight 488

Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity.  ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research. 

Dylight 488 can be used to visualize the binding pattern of Allium sativum Lectin (ASA) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that works over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.

Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Glycobiology.

Recommended Usage: 1XPBS can be used for reconstitution/dilution. Recommended dilution 0.5-10 µg /ml.