Amaranthus caudatus Lectin (ACL/ACA) - DyLight 488

Affinity-purified Amaranthus caudatus (ACL/ACA) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the “T” antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.

DyLight 488 is a green-fluorescent dye used to visualize the binding pattern of ACL in cellular imaging and flow cytometry. It performs effectively across a broad pH range (pH 4-9) and offers superior brightness and photostability compared to other green fluorophores, including FITC.

Recommended Usage: Recommended dilutions to use 0.5 – 10 µg / ml in 1XPBS. Less than 0.5 µg will agglutinate neuraminidase treated erythrocytes.

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell sorting/typing, Glycobiology.

References

1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.

2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.