Artocarpus integrifolia Lectin (AIA) - DyLight 488

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein’s structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. This product comes in a stable lyophilized form.

DyLight 488 is a green-fluorescent dye used to visualize the binding pattern of Artocarpus integrifolia (Jacalin) Lectin (AIA) in cellular imaging and flow cytometry. It operates effectively over a broad pH range (pH 4-9) and is brighter and more photostable than many other green-fluorescent dyes, including FITC.

Recommended Usage: Recommended dilutions 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate rabbit erythrocytes.

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Glycobiology.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. Mohamad SFS et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.: Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. VIJAYAKUMAR T and FORRESTER JA. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.