Artocarpus integrifolia Lectin (AIA) - Semi-Pure

Artocarpus integrifolia Lectin (AIA) is purified by fractionation and has a tetrameric two-chain structure consisting of four identical protomers, with an isoelectric point of pH 7.8. This lectin exhibits specificity for blood group types O (+SA) and T. Jacalin is classified as a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification provides Jacalin with a novel carbohydrate-binding site at the N terminus of the α-chain. The carbohydrate-binding specificity of AIA for the Thomsen-Friedenreich antigen (TF-Galß1-3GalNAc) suggests antitumor potential, as this antigen is present in over 85% of human carcinomas. Jacalin has demonstrated antiproliferative effects on colonic epithelial cells when administered to carcinogen-exposed mice, indicating potential in colorectal cancer treatment. Possible anticancer pathways include COX-2 inhibition and apoptotic or necrotic cell death induced by high levels of TNF-α from Jacalin-activated macrophages. Additionally, Jacalin may be explored in SARS-CoV2 research, as it can recognize the receptor-binding domain (RBD) of the virus and induce a conformational change. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal, and 2-deoxy-α-D-galactose. This product is available in a stable lyophilized form.

Recommended Usage: Recommended dilutions of 10-60 µg / ml in 1XPBS. Less than 1 mg / ml can agglutinate neuraminidase treated by human erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Mitogenic stimulation. Immunocytochemistry. Cell typing. Glycobiology.

References

1. Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
2. S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
3. Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
4. Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
5. T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.