Concanavalin A Lectin (Con A) - DyLight 488

Concanavalin A Lectin (Con A) is isolated from jack bean seeds (Canavalia ensiformis) and purified by affinity chromatography. Con A is composed of identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues) and while above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. It has a carbohydrate specificity towards α-mannose and α-glucose and elutes with MeαMan + MeαGlc. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells which aggregate by Con A; normal white cells do not. This product comes in a stable lyophilized form

DyLight 488 can be used to visualize the binding pattern of Concanavalin A (Jackbean) lectin (Con A) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that functions effectively over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.

Recommended usage: Recommended dilutions of 0.5 – 10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. More than 15 μg/ml can agglutinate rabbit/human/porcine erythrocytes.

Application

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, ELLA, Blotting, Bioconjugations, Cancer research & study, Glycobiology.

References

1.R Darmani H, Coakley WT. Contact patterns in concanavalin A agglutinated erythrocytes. Cell Biophys. 1991 Feb;18(1):1-13.
2.Olson MO, Liener IE. Some physical and chemical properties of concanavalin A, the phytohemagglutinin of the jack bean. Biochemistry. 1967 Jan;6(1):105-11.
3.McCubbin WD, Kay CM. Molecular weight studies on concanavalin A. Biochem Biophys Res Commun. 1971 Jul 2;44(1):101-9.