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Description

Dylight 488 can be used to visualize the binding pattern of Griffonia simplicifolia Lectin (GSL I+II) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that works over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Appearance color

Off-white to yellow to green

Appearance form

Liquid

Molecular Weight

113 kDa

Abbreviation (Lectins Only):

GSL I+II

Source

Bandeiraea

Blood Group Specificity

A(-SA), B

Carbohydrate Specificity

GalNAc, Galactose, GlcNAc

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

DyLight® 488

Inhibitory Carbohydrate

Methyl α-D-Galactopyranoside, Melibiose, N,N'-Diacetylchitobiose

Fluorescence

Green

Divalent Ions

Ca++, Mn++

Excitation

493 nm

Mitogenic Activity

Emission

518 nm