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11140006 bioPLUS™

Hot Firepol® (TAQ)

	SKU	Pack Size	Price
	11140006-1	1000 Unit(s)	$202.70
	11140006-2	4000 Unit(s)	$524.87

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Hot Start Themostable DNA Polymerase HOT FIREPol® is a high quality enzyme that can be used in various scientific technologies in the gene technology field. e.g. automatic hot start primer extension.
Inactive at ambient temperatures and is activated by a 15-minute incubation at 95-97°C
Incubation prevents extension of nonspecifically annealed primers formed at low temperatures.
Enzyme has 5' to 3' polymerization-dependent exonuclease replacement activity but lacks a 3' to 5' exonuclease activity.
Enzyme has the "extendase activity" allowing TA cloning.
Source: Purified from E. Coli strain.
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10mmole of dNTP into an acid-insoluble form in 30 minutes at 74°C.
Concentration: 5U/µL
Storage and Dilution Buffer: 50% glycerol, 20mM Tris-HCl pH 8.7 at 25°C, 1000mM KCl, 0.1mM EDTA and stabilizers.