Human Fibronectin

Introduction

Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related to venous thromboembolism (VTE), particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE. Fibronectin plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin consists of two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulfide-linked dimer in the plasma. Hepatocytes produce the plasma form, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, and certain epithelial cells. Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different extracellular matrix components and membrane-bound Fibronectin receptors on cell surfaces.

Description

Human fibronectin is produced and purified from human plasma, having a molecular weight of 440kDa.

Source

Human Plasma.

Physical Appearance

Filtered White lyophilized (freeze-dried) powder.

Formulation

Fibronectin was lyophilized from a nonsterile 2mg/ml buffer of 10 mM sodium phosphate, pH 7.5, and 0.15M NaCl.

Solubility

We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a 0.2mg/ml concentration using sterile water. Let stand for 1-2 hours. The recommended concentration is 4M-5M urea.

When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).

Stability

Although lyophilized Fibrin is stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution, Fibrin should be stored at 4°C between 2-7 days and for future use below -18°C.

Purity

≥ 95.0% as determined by SDS-PAGE.

Usage

This product is for laboratory research use only. It may not be used as a drug, agricultural or pesticidal product, food additive, or household chemical.