Close

Description

Dylight 488 can be used to visualize the binding pattern of Phaseolus vulgaris Lectin (PHA-L) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that works over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.

Properties

Shelf life

2 years

Storage Temperature

-20°C

ECCN #

EAR99

Appearance color

Off-white to yellow to green

Appearance form

Liquid

Molecular Weight

130 kDa

Abbreviation (Lectins Only):

PHA-L

Source

Red Kidney Bean

Blood Group Specificity

Human, Rabbit Leukocytes

Carbohydrate Specificity

Galactose

Storage Buffer

20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2, 5% Glycerol, 0.1% D-Trehalose Dihydrate, 0.01% Sodium Azide

Conjugate/Tag/Matrix

DyLight® 488

Inhibitory Carbohydrate

Neu5Acα2-3Galβ1-4GlcNAcβ1-2Man

Fluorescence

Green

Divalent Ions

Ca++, Mn++

Excitation

493 nm

Mitogenic Activity

Yes

Emission

518 nm