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Description

For the rapid isolation of inclusion body proteins

  • All-in-one solution for inclusion body protein isolation and purifcation
  • Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins
  • Fast and convenient spin column protocol

FEATURES AND BENEFITS

  • Three kits in one for acidic and basic proteins - The kit provides essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography. The kit includes solutions and protocols for use with either acidic or basic proteins.
  • Gentle disruption procedure provides high purity final product - Cell lysis is accomplished through non-ionic detergent chemical disruption. The use of a needle and syringe reduces viscosity and facilitates purification, producing protein often >95% pure. Proteins are then ready for SDS-PAGE, 2D gels, mass spectrometry analysis or other procedures.
  • Convenient process volume - Analyze 100 mL of microbial cell culture yielding 12 mg of protein.
  • Long shelf life - The kit uses an enzyme-free process with stable solutions for longer shelf life.
  • Fast process time - Process time is about 2 hours for a single sample.
  • Ready-to-use columns - No column preparation is required.

Properties

Shelf life

2 years

Storage Temperature

RT