BCA Protein Assay Reagent A

The BCA Protein Assay, or Bicinchoninic Acid Assay, is used for protein quantification. Reagent A is the first step in measuring the production in whole-cell lysates and affinity-column fractions. BCA Protein Assay Reagent A provides a greater protein-to-protein uniformity because it is not as affected by compositional differences.

This alkaline reagent is manufactured using only Biotechnology Grade raw materials. In order to perform the assay, Reagent A should be mixed at a ratio of 50:1 with BCA Protein Assay Reagent B. Samples are then incubated in the working solution for 30 minutes at 37°C or 2 hours at ambient temperature.

For a simple step-by-step procedure, purchase bioWORLD's complete BCA Protein Assay with BSA Standards. We carry all of the necessary reagents for protein purification and electrophoresis.

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

References

See "Quantifying protein by bicinchoninic Acid" on PubMed.