Autophagy Related 5 Human (Recombinant)

Synonyms

Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, ASP, APG5, APG5L, hAPG5.

ATG5 is a member of the autophagy-related (ATG) genes family. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. ATG5 protein is essential for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. In addition ATG5 takes a vital part in the apoptotic process and it is expressed relatively late in the apoptotic process, downstream of caspase activity.

Description

ATG5 produced in E. coli is a single, non-glycosylated polypeptide chain containing 295 amino acids (1-275a.a.) and having a molecular mass of 34.6kDa. ATG5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered clear solution.

Formulation

APG5 at a concentration of 0.25mg/ml containing 20mM Tris, pH 8.0, 1mM DTT , 0.2M NaCl and 40% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please prevent freeze-thaw cycles.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLPRVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNITVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLKEVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.