B-Cell Leukemia/Lymphoma 2 Human (Recombinant) (-BH3)

Synonyms

Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.

BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Description

Bcl-2 Des BH3 domain (93-107 residues) Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 and 108-218. The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Source

Escherichia coli

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

Solubility

Suspend Bcl-2 in 100µl of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

Stability

Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Applications

Input marker or positive control (Western Blotting).
Function study.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

Bcl 2 Human, Bcl 2 Human (minus BH1 domain), Bcl 2 Human (minus BH2 domain), Bcl 2 Human (minus BH4 domain), Bcl 2 Human (minus NWGR domain), BCL2 Antibody, BCL2 Human, His