Bovine Phosphoinositide 3-kinase alpha p110a/p85a (Recombinant)

Recombinant bovine Phosphoinositide 3-kinase alpha p110a/p85a (PI3Ka)

Source: Sf9 insect cells.

Description: PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3\'-phosphorylated phosphoinositides. PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation. The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Recombinant Bovine Phosphoinositide 3-kinase alpha is a glycosilated protein having a molecular weight as follows: p85a chain 83.5 kDa, p110 chain 124.3 kDa.

Physical Appearance: Sterile Filtered clear soultion.

Formulation: 25 mM HEPES, pH 8.0, 25 mM NaCl, 2.5 mM MgCl2 and 50% glycerol.

Storage: Recombinant bovine PI3Ka although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90.0% as determined by SDS-PAGE.

Biological Activity: 1000 U/mg

Unit Definition: 1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.